Lake Manatee is located in Manatee County in southwestern Florida in the United States. Highway 64 crosses the eastern end of the lake, while the Lake Manatee State Park is found at the southwestern end. The Manatee River flows into the lake at the eastern end, then continues to the west, where it flows into Tampa Bay. Lake Manatee is a major source of water for Bradenton and other cities in Manatee County, providing 32 million gallons per day.